The street runs from Cornmarket to the Saint James's Gate Brewery, where Guinness is brewed; there Thomas Street connects with James's Street.
The National College of Art and Design is located on Thomas Street, as is John's Lane Church, which has the highest steeple in the city, Vicar Street (music venue), Michelin-starred restaurant Variety Jones, St. Catherine's church where the patriot Robert Emmet was executed, as well as The Thomas House bar and venue.
Thomas Street is one of only four streets in Dublin where street trading is permitted (the other three being Wexford Street, Henry Street, and Moore Street).